| Registro completo |
| Provedor de dados: |
Biological Sciences
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| País: |
Brazil
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| Título: |
An anti-dengue and anti-herpetic polysulfated fraction isolated from the coenocytic green seaweed Caulerpa cupressoides inhibits thrombin generation in vitro
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| Autores: |
Rodrigues, José Ariévilo Gurgel
Eloy, Ygor Raphael Gomes
Vanderlei, Edfranck de Souza Oliveira
Cavalcante, Jéssica Figueiredo
Romanos, Maria Tereza Villela
Benevides, Norma Maria Barros
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| Data: |
2017-06-16
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| Ano: |
2017
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| Palavras-chave: |
Química de macromoléculas Bryopsidales
Marine glycans
Cytotoxicity
Viral infection
Thrombin. Bioprospecção
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| Resumo: |
The antiviral potency of Caulerpa cupressoides (Chlorophyta) sulfated polysaccharidic fractions (Cc-SP1→3) associated with thrombin generation (TG) assay is unknown. This study analyzed the structure of Cc-SP1 and its effects against herpes viruses (HSV-1 and HSV-2), dengue-virus (DENV-1), human-metapneumovirus (HMPV) and adenovirus (AdV) in Vero and mosquito C6/36 cell-lines and as inhibitor of TG in 60-fold diluted human plasma using continuous system. Infrared analysis indicated ulvan containing 11% sulfate, 40.16% total sugars and 6% uronic acid. Procedures of agarose/polyacrylamide gels electrophoresis revealed two major components presenting sulfate and non SPs, with molecular dispersion ranging from 8 to > 100 kDa, respectively, as confirmed by gel permeation chromatography. Cc-SP1 did not cause cytotoxicity up to 1000 μg mL-1 and was more effective inhibitor of DENV-1 (96%, 0.35 μg mL-1) compared with HSV-1 (90%, 27 μg mL-1) and HSV-2 (99.9%, 0.9 μg mL-1) in Vero cell-line, whose selectivity indexes were > 714, > 9.2 and > 131, respectively, but was inactive against HMPV, AdV and C6/36 cell-line using plate reduction assay. Cc-SP1 required concentration 20.8-fold higher of SPs than heparin for abolishes intrinsically TG, but at concentrations so far the anti-HSV-1. Therefore, TG assay provides data to guide studies of Cc-SP1 on anticoagulant/antiviral effects.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28081
10.4025/actascibiolsci.v39i2.28081
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| Editor: |
Universidade Estadual De Maringá
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| Relação: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28081/pdf
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| Formato: |
application/pdf
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| Fonte: |
Acta Scientiarum. Biological Sciences; v. 39, n. 2 (2017); 149-159
Acta Scientiarum. Biological Sciences; v. 39, n. 2 (2017); 149-159
1807-863X
1679-9283
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| Direitos: |
Direitos autorais 2017 Acta Scientiarum. Biological Sciences
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